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anti cd40  (Miltenyi Biotec)
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Miltenyi Biotec anti cd40
Anti Cd40, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse cd40 antibody  (MedChemExpress)
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The <t>CD40</t> agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant
Anti Mouse Cd40 Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd40+antibody/Anti-Mouse+CD40+Antibody/pmc13429555-23-8-20
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cd40 antibody, anti-human  (Miltenyi Biotec)
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The <t>CD40</t> agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant
Cd40 Antibody, Anti Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd40 antibody, anti-human - by Bioz Stars, 2026-10
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cd40 antibody  (Miltenyi Biotec)
96
Miltenyi Biotec cd40 antibody
The <t>CD40</t> agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant
Cd40 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/bio_rxiv__64898__2026__06__05__730365-105-10-12
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agonistic antibodies against cd40  (Miltenyi Biotec)
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Miltenyi Biotec agonistic antibodies against cd40
CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human <t>CD40</t> and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.
Agonistic Antibodies Against Cd40, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/pmc13275367-71-11-20
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anti cd40 blocking antibody  (Miltenyi Biotec)
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CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human <t>CD40</t> and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.
Anti Cd40 Blocking Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/10__1016_slash_j__isci__2026__116175-342-29-32
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anti cd40 blocking antibody - by Bioz Stars, 2026-10
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anti human miltenyi biotec cat  (Miltenyi Biotec)
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CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human <t>CD40</t> and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.
Anti Human Miltenyi Biotec Cat, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/pm42167232-239-38-39
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anti human miltenyi biotec cat - by Bioz Stars, 2026-10
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cd40 blocking antibody  (Miltenyi Biotec)
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Miltenyi Biotec cd40 blocking antibody
CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human <t>CD40</t> and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.
Cd40 Blocking Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The CD40 agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant

Journal: Annals of Hematology

Article Title: Identification and validation of a prognostic risk-scoring model in drug-resistant ALL and evaluation of immune micro-environment

doi: 10.1007/s00277-026-07080-3

Figure Lengend Snippet: The CD40 agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant

Article Snippet: Phorbol 12-myristate 13-acetate (PMA, HY-18739), Daunorubicin hydrochloride (HY-13062), anti-mouse CD40 antibody (FGK45, HY-P990680) and anti-Human PD-L1 (HY-108730) were obtained from MedchemExpress.

Techniques: CCK-8 Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Injection, Control

CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human CD40 and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.

Journal: Frontiers in Immunology

Article Title: Staphylococcus aureus serine protease-like protein B elicits a type 1/type 2 immune response in atopic dermatitis patients

doi: 10.3389/fimmu.2026.1798583

Figure Lengend Snippet: CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human CD40 and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.

Article Snippet: To prevent CD154 internalization and provide co-stimulation and a survival signal, agonistic antibodies against CD40 (1 μg/mL, 130-094-133, clone HB14, Miltenyi, Bergisch Gladbach, Germany) and CD28 (0.5 μg/mL, 555726, clone CD28.2, Becton Dickinson GmbH, Franklin Lakes, NJ, USA) were added for 24 h. To investigate SplB-specific T cells, PBMCs were stained with antibodies (unless otherwise stated: from BioLegend, San Diego, CA, USA): PerCP-conjugated anti-human CD4 (317432, clone OKT4) or PE-Cy5-conjugated anti-human CD4 (555348, clone RPA-T4, Becton Dickinson GmbH, Franklin Lakes, NJ, USA) BV510-conjugated anti-human CD14 (301842, clone M5E2), BV510-conjugated anti-human CD19 (302242; clone HIB19), FITC-conjugated anti-human CD154 (310804; clone 24-31), APC-conjugated anti-human CXCR3 (FAB160A, clone 49801, R&D Systems Inc, Minneapolis, MN, USA), BV421-conjugated anti-human CCR4 (359414, clone L291H4), PE-Cy7-conjugated anti-human CCR6 (353418, clone G034E3), and PE-conjugated anti-human CCR10 (130-120-407, clone: REA326, Miltenyi, Bergisch Gladbach, Germany) ( ).

Techniques: Isolation, Expressing, Control, MANN-WHITNEY, Comparison